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Novel DNA diagnostic technology in plant disease control
Reference
JRI07333
Principal Investigator / Supervisor
Dr Derek Hollomon
Co-Investigators /
Co-Supervisors
Institution
Rothamsted Research
Department
Sustainable Soils and Grassland Systems
Funding type
Research
Value (£)
14,396
Status
Completed
Type
Research Grant
Start date
01/02/1997
End date
01/08/1997
Duration
6 months
Abstract
DNA-based diagnostic systems offer considerable potential to improve leaf blotch (Septoria tritici) control through more accurate pre-symptomatic detection. This project aims to develop generic, microtitre based diagnostic technologies for the detection of plant diseases, using the beta-tubulin gene as the target. It will focus on ways to maximise capture of PCR products in oligonucleotide modified microtitre wells, and how to maximise hybridisation of labelled probes to this PCR product. Several approaches will be explored to generate a single stranded PCR product including use of Phosphorothionate containing PCR primers to restrict the activity of 5' exonuclease to ensure complete digestion of just one strand of the PCR product.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Joint Equipment Research Initiative 1996 (JE1) [1996]
Funding Scheme
X – not Funded via a specific Funding Scheme
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