Award details

(A) Structural and mechanistic studies of bio- molecules involved in plant growth development and defence

Reference	JRE10849
Principal Investigator / Supervisor	Professor Michael Beale
Co-Investigators / Co-Supervisors	Professor Peter Hedden, Dr Andrew Phillips
Institution	Rothamsted Research
Department	Sustainable Soils and Grassland Systems
Funding type	Research
Value (£)	57,044
Status	Completed
Type	Research Grant
Start date	01/05/1999
End date	01/01/2000
Duration	8 months

Abstract

The refurbished NMR will be used in multidisciplinary projects aimed at characterisation and exploitation of the molecular mechanisms of plant hormone biosynthesis and action. Specifically, this will involve the analysis of isotope-labelled mechanisms of plant hormone biosynthesis and action. Specifically, this will involve the analysis of isotope-labelled gibberellins, and gibberellin analogues including affinity probes, and the use of these compounds to study the structure and mechanisms of the gibberellin dioxygenases, three of which have been cloned and expressed in E. coli. These experiments will include observation of the fate of isotope-labelled atoms during enzyme turnover. NMR will also be used to support synthetic and structural work aimed at understanding the mode of action of plant hormones. In particular, this involves the production and use of affinity labels for gibberellin- and abscisic acid-binding proteins and the development of single-chain antibody/green fluorescent protein conjugate technology as tools for the assay of ligands both in vitro and in planta. The instrument will also contribute significantly to a study of the structure and active conformation of jasmonate and its role in plant defence. It will also be used for collaborative projects on the structure and determination of other natural products induced in plants as part of the defence mechanism.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Research Equipment Initiative 1998 (JE3) [1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

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