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(A) Kinetic analysis of enzymes by stopped-flow spectrometry
Reference
JRE10837
Principal Investigator / Supervisor
Krebs Professor of B Christopher Hunter
Co-Investigators /
Co-Supervisors
Institution
University of Sheffield
Department
Molecular Biology and Biotechnology
Funding type
Research
Value (£)
65,028
Status
Completed
Type
Research Grant
Start date
01/01/1999
End date
01/07/1999
Duration
6 months
Abstract
The objectives are to conduct pre-steady state measurement on a wide variety of enzymes, spanning measurements on tetrapyrrole, isoprenoid, RNA, DNA and haem substrates and ligands, and requiring the use of a stopped-flow spectrometer for measurement of transient fluorescence, absorbance and CD signals. (i) Professor C H Hunter: Kinetic analysis of the chlorophyll and carotenoid biosynthetic pathways; (ii) Dr J A Grasby: stopped flow kinetics of the hairpin ribozyme monitored by fluorescence of 2-amino purine; (iii) Drs Jon Waltho and Rosie Stanforth: cystatin C folding and amyloidogenesis, monitoring of PGK folding intermediates; (iv) Professor Robert Poole: the function of the haemoglobin-like protein (HMP) of Escherichia coli; Dr I A Murray: pre-steady state kinetic analyses of CoA- dependent-acyltransferases; (v) Dr D P Hornby: conformational transitions that accompany DNA recognition and catalysis by DNA methyltransferases; (vi) Dr D Kelly: ligand- binding kinetics and conformational thermodynamics of a novel bacterial periplasmic binding-protein dependent transport system, the function and interactions of chemotaxis proteins from the human gastric pathogen Helicobacter pylori.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Joint Research Equipment Initiative 1998 (JE3) [1998]
Funding Scheme
X – not Funded via a specific Funding Scheme
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