Award details

(A) Proteome analysis of the Streptomyces Arabidopsis E. coli and Aspergillus genomes using MALDI-TOF spectrometry

Reference	JRE10832
Principal Investigator / Supervisor	Dr Michael Naldrett
Co-Investigators / Co-Supervisors	Professor Mervyn Bibb
Institution	John Innes Centre
Department	Disease and Stress Biology
Funding type	Research
Value (£)	97,200
Status	Completed
Type	Research Grant
Start date	16/07/1999
End date	16/01/2000
Duration	6 months

Abstract

Investigation of the protein complements of the genomes of Streptomyces coelicolor, Arabidopsis thaliana, Escherichia coli and Aspergillus fumigatus at the JIC requires the addition of a high sensitivity, high mass accuracy mass spectrometer with robot. MALDI-TOF combines these attributes with ease of use and high throughput. The reliable identification of approximately 80% of proteins from a sequenced genome can be achieved by searching the masses of an experimentally-derived tryptic digest of a protein (isolated by two-dimensional gel electrophoresis) against a mass database of theoretical digest fragments derived from the genome sequence. It is now possible to relate global changes in protein patterns during growth, stress, differentiation or other physiological changes to the genome itself. MALDI-TOF is an essential tool for analysis of proteomes. MALDI-TOF will also contribute to more than fifteen other areas of research at JIC including agri-food, biotechnology, biomimetic chemistry, nitrogen fixation and x-ray crystallography. Projects throughout the Norwich Research Park will also find this useful tool for general analysis. A substantial financial contribution has been given by PE-Biosystems to further joint interests in a) techniques for plant proteomics, b) carbohydrates and glycoproteins and c) development of novel electropolymer surface chemistries for new approaches to proteomics.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Research Equipment Initiative 1998 (JE3) [1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

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