Award details

(A) A central DNA sequencing facility for the school of biological sciences

Reference	JRE10830
Principal Investigator / Supervisor	Professor John Turner
Co-Investigators / Co-Supervisors
Institution	University of East Anglia
Department	Biological Sciences
Funding type	Research
Value (£)	64,208
Status	Completed
Type	Research Grant
Start date	01/09/1999
End date	01/03/2000
Duration	6 months

Abstract

The application, made on behalf of researchers in the School of Biological Sciences, is for a contribution towards the purchase of an ABI PRISM 377 DNA sequencer. The sequencer will be run by a dedicated technician, and will provide the central facility in this School for the determination of DNA sequence and for analysis of microsatellite and AFLP markers in populations of fish, animals, and plants. The new DNA sequencing facility will underpin 27 current projects supported by grants exceeding irectorate11M from the BBSRC, MAFF, industry, charities and the MRC. These include projects to isolate and to characterise bacterial genes for metalloproteins and related enzymes involved in electron transport in the nitrogen cycle, sulphur cycle and respiration, bacterial genes for colicin production, Arabidopsis genes involved in signalling and defence, mammalian genes for metalloproteinases and their inhibitors involved in cancer and development, and analysis of molecular markers in studies on populations of fish, mammals, and plants. These projects formed an important part of this Schools last RAE submission, which was rated 5. The ABI sequencer will therefore play a vital role in supporting these projects for RAE 2001. The ABI sequencer will remove our present dependence upon outside contractors for DNA sequence determination, which have proven slow, expensive, or inflexible. It will also replace our two ALF DNA sequencers, which have been removed from operation because these are both unreliable and inefficient. The ABI sequencer will meet our current and projected requirement for the analysis of approximately 180 samples each week rising to approximately 600 per week by the year 2003.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Research Equipment Initiative 1998 (JE3) [1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

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