Award details

(A) Imaging of cholorophyll fluorescence in Arabidopsis seedlings for mutant analysis and large-scale screening

Reference	JRE10813
Principal Investigator / Supervisor	Professor Brian Gordon Forde
Co-Investigators / Co-Supervisors	Professor Tony Miller
Institution	Rothamsted Research
Department	Plant Biology & Crop Science
Funding type	Research
Value (£)	26,772
Status	Completed
Type	Research Grant
Start date	29/04/1999
End date	29/10/1999
Duration	6 months

Abstract

We propose to develop and apply a non-invasive screening method for monitoring nutrient stress simultaneously in large numbers of wild-type, mutant and transgenic Arabidopsis seedlings. A high resolution CCD camera fitted with an image intensifier will be used to obtain high resolution false-colour images of chlorophyll fluorescence. Measurements of chlorophyll fluorescence have previously been shown to provide a sensitive means of detecting early signs of nutrient stress in plants, the sensitivity being greatly increased by high resolution imaging rather than averaging the emissions over a whole leaf. In the first part of the work the imaging system will be used to investigate the sensitivity of a number of existing Arabidopsis mutants and transgenics that are altered in various aspects of nutrient transport or metabolism. In the second part, it will be used to screen chemically mutagenised and T-DNA- or transposon or metabolism. In the second part, it will be used to screen chemically mutagenised and T-DNA- or transposon-tagged populations of Arabidopsis seedlings for mutants that are more (or less) sensitive to short-term nutrient stress than the wild-type. The aim is to provide an effective and non-destructive means of monitoring the physiological status of the plant and so contribute to the current efforts to exploit the information and resources emanating from in the Arabidopsis genome project.

Summary

unavailable

Committee	Not funded via Committee
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Research Equipment Initiative 1998 (JE3) [1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search