Award details

(A) Expression and localisation of cellular proteins

Reference	JRE10806
Principal Investigator / Supervisor	Professor John Hickman
Co-Investigators / Co-Supervisors	Dr Gerard Brady, Dr Peter Brown, Professor Keith Caldecott, Professor Caroline Dive, Professor Daniela Riccardi
Institution	The University of Manchester
Department	Life Sciences
Funding type	Research
Value (£)	30,000
Status	Completed
Type	Research Grant
Start date	01/04/1999
End date	01/10/1999
Duration	6 months

Abstract

A versatile, high quality fluorescence microscope and image package is required to examine the expression and subcellular localisation of proteins involved in: a) DNA damage repair. (we will determine how cells detect DNA damage in S phase and how they respond to this damage); b) The regulation of apoptosis. (we will investigate changes in the subcellular location of the Bcl-2 family, the role of a novel intracellular Ca2+ -sensing receptor in signalling for apoptosis and molecular events associated with commitment to this programme of cell death); c) Ion and water transport. (we will investigate cellular distribution of ion channels and receptors in the brain and in the smooth muscle and the distribution of water channels in the epithelia of exocrine glands). Acquisition of this microscope will promote integrated multidisciplinary approaches. Complementary fluorescence microscopy and flow cytometric studies will be conducted in parallel to address questions of cellular location, expression level and changes in the conformation of proteins of interest. In addition, as described below, the microscope will stimulate collaborations between the applicants.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Research Equipment Initiative 1998 (JE3) [1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

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