Award details

(A) High sensitivity and rapid throughput protein characterisation using robotic Maldi TOF

Reference	JRE10781
Principal Investigator / Supervisor	Professor Antony Slabas
Co-Investigators / Co-Supervisors	Professor Robert Edwards, Dr Nicholas Hole, Dr Paul Nicholas Hunt, Professor Keith Lindsey
Institution	Durham University
Department	Biological and Biomedical Sciences
Funding type	Research
Value (£)	120,000
Status	Completed
Type	Research Grant
Start date	11/02/1999
End date	11/08/1999
Duration	6 months

Abstract

We aim to use an automated MALDI TOF facility to investigate biological problems in the areas of: 1. Identification of protein compartments of specific subcellular components and/or cell types. 2. Identification of proteins which are altered in level/covalently modified in response to elicitors. 3. 2D- electrophoresis to identify changes in proteins in selected Arabidopsis mutants. 4. Identification of amino acid residues involved in enzyme catalytic mechanism/substrate selectivity and analyses of samples generated for crystallography. The purpose of this equipment is essential to allow for high selectivity, high throughput protein analysis which is beyond the capability of the current Department Edmon Sequencer.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Research Equipment Initiative 1998 (JE3) [1998]
Funding Scheme	X – not Funded via a specific Funding Scheme

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