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BBSRC David Phillips Fellowship A study into the physical properties of the fission yeast myosins

ReferenceJF20605
Principal Investigator / Supervisor Professor Daniel Mulvihill
Co-Investigators /
Co-Supervisors
Institution University of Kent
DepartmentSch of Biosciences
Funding typeResearch
Value (£) 394,712
StatusCompleted
TypeFellowships
Start date 01/10/2003
End date 30/09/2008
Duration60 months

Abstract

To correlate the in vitro biochemical and biophysical properties of these myosins with observations from established in vivo assays to advance our understanding of myosin function in general. The unique aspect of this project is the exploitation of fission yeast genetics allowing precise gene replacement of wild type molecules with those that have been mutated to alter the biophysical/biochemical properties in a defined manner. We will use fast kinetic methods to measure the ATPase and motor activities of microgram quantities of protein on a millisecond time scale in vitro and introduce mutants in which these parameters are altered.

Summary

unavailable
Committee Closed Committee - Biochemistry & Cell Biology (BCB)
Research TopicsMicrobiology, Structural Biology
Research PriorityX – Research Priority information not available
Research Initiative Fellowship - David Phillips Fellowship (DF) [1995-2015]
Funding SchemeX – not Funded via a specific Funding Scheme
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