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Characterisation of the signalling pathway involving phosphatidylinositol (345) trisphosphate binding protein centaurin-alpha
Reference
JF13142
Principal Investigator / Supervisor
Professor Venkateswarlu Kanamarlapudi
Co-Investigators /
Co-Supervisors
Institution
University of Bristol
Department
Biochemistry
Funding type
Research
Value (£)
242,768
Status
Completed
Type
Fellowships
Start date
01/01/2000
End date
01/01/2005
Duration
60 months
Abstract
The objective of this proposal is to understand the physiological role of centaurin-alpha and its regulation by PIP3. I propose to do this using the following strategies. Firstly, using both biochemical and molecular biological approaches I will characterise the in vitro Arf GAP activity and PIP3-binding ability of centaurin-alpha. In addition, I will use the yeast two- hybrid system not only to identify the specific Arf isoform(s) which interact with centaurin- alpha but will also characterise other proteins which interact with centaurin- alpha. The information which I will obtain from these methods will be used to identify the in vivo signalling in which centaurin-alpha is involved. Finally, I will investigate the physical structure of centaurin-alpha. The main targets are as follows: Year 1-2: Biochemical and mutational analysis of the Arf GAP activity and PIP3-binding to recombinant centaurin- alpha. Identification of centaurin-alpha specificity for Arfs using yeast two- hybrid system and isolation of full-length cDNA for the novel centaurin-alpha binding kinesin like protein (KLP). Year 3-4: Functional and molecular analysis of KLP, in vivo functional analysis of centaurin-alpha and initiation of structural analysis; Year 5: Completion of in vivo functional and structural analysis.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Fellowship - David Phillips Fellowship (DF) [1995-2015]
Funding Scheme
X – not Funded via a specific Funding Scheme
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