Award details

The role and regulation of photosynthesis around vascular tissue

Reference	JF13138
Principal Investigator / Supervisor	Professor Julian Hibberd
Co-Investigators / Co-Supervisors
Institution	University of Cambridge
Department	Plant Sciences
Funding type	Research
Value (£)	236,616
Status	Completed
Type	Fellowships
Start date	01/01/2000
End date	01/01/2005
Duration	60 months

Abstract

The aim of the project is to investigate the role and regulation of photosynthesis in selected cell-types in plant stems using enhancer trap lines of Arabidopsis thaliana. Photosynthetic organs contain many different cell-types containing chloroplasts and functional photosynthetic apparatus. At present we do not know if photosynthesis within distinct cell- types plays specific functions. I will determine the role and regulation of photosynthesis in different cell-types of plant stems. To achieve this I will use the enhancer trap lines to control expression of antisense mRNA constructs to photosynthetic proteins, thus decreasing photosynthesis in discrete cell-types of plant stems. I aim to understand the role and regulation of photosynthesis in cells close to the vasculature. This will be achieved by: (1) Producing the antisense constructs to RbcS driven by a minimal 35S promoter and GAL4- VP16 through upstream activated sequence (3 months). (2) Transforming A. thaliana with these constructs, and then crossing these plants into existing enhancer trap lines with targeted gene expression within specific cell-types (24 months; overlapping with (1), (3) & (4). (3) Using the enhancer trap lines to determine the photosynthetic capacity of cells close to the vasculature (12 months). (4) Using enhancer trap lines with reduced photosynthetic ability in specific cell-types to determine the role of photosynthesis in these cell-types, and, the control of carbon flux within the plant (24 months; overlapping with (5). (5) To investigate heterogeneity in the regulation of photosynthetic gene expression by sugars (24 months; with (4)).

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Fellowship - David Phillips Fellowship (DF) [1995-2015]
Funding Scheme	X – not Funded via a specific Funding Scheme

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