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Structural analysis of the bacterial MutHLS and UvrABC DNA-repair complexes

Reference	JF11443
Principal Investigator / Supervisor	Dr Tracey Barrett
Co-Investigators / Co-Supervisors
Institution	Birkbeck College
Department	Biological Sciences
Funding type	Research
Value (£)	251,216
Status	Completed
Type	Fellowships
Start date	01/10/1998
End date	01/10/2003
Duration	60 months

Abstract

The objective of this proposed research programme is to obtain a structural basis for the key protein-protein and protein-DNA interactions involved in the recognition and repair of DNA damage in both the MutHLS and UvrABC DNA repair pathways. Clones are currently available in the host institution for MutL, MutS (provided by Professor M Marinus) and the UvrABC proteins (provided by Professor A Sancar). Years one and two of the fellowship would involve establishing conditions for the large scale expression purification and crystalisation of these proteins together with relevant complexes. In the latter part of the fellowship the three-dimensional structure determination of these proteins and complexes would be performed in addition to the design and analysis of site directed mutants to further examine the various mechanisms involved in target recognition and catalysis.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Fellowship - David Phillips Fellowship (DF) [1995-2015]
Funding Scheme	X – not Funded via a specific Funding Scheme

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