Award details

Molecular neurogenetics of circadian clocks in higher eukaryotes

Reference	JF11258
Principal Investigator / Supervisor	Professor Ezio Rosato
Co-Investigators / Co-Supervisors
Institution	University of Leicester
Department	Genetics
Funding type	Research
Value (£)	249,712
Status	Completed
Type	Fellowships
Start date	01/10/1998
End date	01/10/2003
Duration	60 months

Abstract

The objectives of the proposed research programme are: a) To perform a comparative study of the interactions of the clock genes period (per) and timeless (tim) in Drosophila melanogaster and Musca domestica, using newly developed in vivo reporter genes, and to extend the use of these reporters for the study of postranslational modifications in the two clock proteins. b) To develop a novel in vivo two-hybrid system in Drosophila using phage T7 RNA polymerase, the yeast GAL4 activator, and the GFP reporter. The first two years will be spent isolating the tim gene in M. domestica, characterising it, generating tim-gfp and per-gfp fusion genes and studying their expression. As this work progresses, the third year will also be used to initiate the postranslational study of PER and TIM using FRET. By the fifth year it is hoped to have at least initiated work with the in vivo Drosophila two- hybrid system. Some of this effort will be shared with a postgraduate student that the department will make available in the first or second year.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Fellowship - David Phillips Fellowship (DF) [1995-2015]
Funding Scheme	X – not Funded via a specific Funding Scheme

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