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BBSRC David Phillips Research Fellowship: Nitric oxide and the control of epithelial fluid transport
Reference
JF07287
Principal Investigator / Supervisor
Professor Shireen Davies
Co-Investigators /
Co-Supervisors
Institution
University of Glasgow
Department
Molecular Genetics
Funding type
Research
Value (£)
213,084
Status
Completed
Type
Fellowships
Start date
01/07/1996
End date
01/01/2002
Duration
66 months
Abstract
Molecular genetic, biochemical and physiological methods will be used to investigate the role of NO signalling and associated signal transducers in epithelial transport function in the Drosophila renal tubule. This is the only in vivo model system presently available in which molecular genetic intervention in the NO/cGMP pathway can be achieved. I propose to:- (i) Investigate the spatial and temporal distribution of components in the NO/cGMP signalling pathways, particularly nitric oxide synthase (NOS), guanylate cyclase and protein kinase G (PKG) in Drosophila renal tubules by in situ hybridisation and immunocytochemistry. (ii) Target pseudosubstrate inhibitors of PKG and constitutively active PKG, to tubule cell sub-types using P[GAL4] enhancer trap mediated gene expression in vivo. (iii) Investigate interactions between cAMP/activated adenylate cyclase, Ca2+ mobilising agents and the NO/cGMP pathway in wild-type and transgenic flies.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Fellowship - David Phillips Fellowship (DF) [1995-2015]
Funding Scheme
X – not Funded via a specific Funding Scheme
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