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BBSRC Technology Foresight junior research fellowship: exploitation of generic xenobiotic degradation and targeted biotransformation of secondary metabolites using plant p450 cDNAs

Reference	JF07282
Principal Investigator / Supervisor	Dr Simon Warner
Co-Investigators / Co-Supervisors
Institution	Durham University
Department	Biological and Biomedical Sciences
Funding type	Research
Value (£)	23,415
Status	Completed
Type	Fellowships
Start date	01/10/1996
End date	30/06/1997
Duration	9 months

Abstract

General Objectives - to exploit plant p450 genes to: (i) engineer plants to metabolise agrochemical xenobiotics, including herbicides and pesticides and (ii) develop generic approaches for the manipulation of plant secondary metabolism to produce pharmaceutically important chemicals in crop plants and a wealth-creating opportunity. Specific Objectives - (i) To isolate p450 cDNA sequences from tobacco treated with a number of xenobiotics and to investigate utilisation of the specific inducing chemicals as substrates by individual induced p450 enzymes; (ii) To generate transgenic plants with improved ability to perform biotransformation of xenobiotics by increasing the total amount of NADPH p450 reductase and p450 mono-oxygenase concentration; (iii) To predict 3D structure of plant p450 proteins and to construct fusions with the autocatalytic soluble p450BM3 sequence to engineer enzymatic functions of p450 sequences and generate novel soluble p450 proteins (in collaboration with Prof Gordon Roberts, Leicester).

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Fellowship - David Phillips Fellowship (DF) [1995-2015]
Funding Scheme	X – not Funded via a specific Funding Scheme

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