Award details

DNA diagnostic technologies for improving precision in plant disease control

Reference	JEI09428
Principal Investigator / Supervisor	Dr Derek Hollomon
Co-Investigators / Co-Supervisors
Institution	Rothamsted Research
Department	Sustainable Soils and Grassland Systems
Funding type	Research
Value (£)	4,248
Status	Completed
Type	Research Grant
Start date	01/03/1998
End date	01/09/1998
Duration	6 months

Abstract

Advances in molecular biology have provided many new diagnostic opportunities for measurement of plant diseases and monitoring fungicide resistance. Our group is actively engaged in this research, coupled with the identification of molecular mechanisms of fungicide resistance which underpins much of the work. PCR is a cornerstone of many aspects of this research. It is used in our laboratory for the detection and quantitative measurement of specific gene products, DNA sequencing, differential display, marked assisted monitoring using AFLPs, and amplification of DNA fragments for detection of specific DNA base changes using allele specific oligonucleotides (ASO) and single stranded conformational polymorphism (SSCP). To improve the selectivity and sensitivity we need greater versatility in the PCR programmes than is available with our current equipment. We also need higher throughput and to be able to use smaller volumes.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Research Equipment Initiative 1997 (JE2) [1997]
Funding Scheme	X – not Funded via a specific Funding Scheme

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