Award details

The use of a cryo-ultramicrotome to study virus assembly

Reference	JEI09411
Principal Investigator / Supervisor	Professor Tom Wileman
Co-Investigators / Co-Supervisors
Institution	The Pirbright Institute
Department	Div of Microbiology Compton
Funding type	Research
Value (£)	31,500
Status	Completed
Type	Research Grant
Start date	31/05/1998
End date	30/11/1998
Duration	6 months

Abstract

We are exploiting the specialised resources of the Pirbright Laboratories to study the assembly of African Swine Fever Virus (ASFV) and Foot and Mouth Disease (FMDV). ASFV is a unique large icosahedral DNA virus that is enveloped by being wrapped by membrane cisternae. FMDV is a picornavirus that sequesters cellular membranes into replication complexes. The replication and assembly of both viruses requires an intimate interaction between viral proteins and host cell membrane compartment and we hope to understand these interactions at a molecular level. We have an urgent need to develop immunogold electron microscopy to identify subcellular membrane compartments accurately, and localise host and viral proteins within them. The high resolution provided by cryosections makes this the method of choice. We have therefore requested funds to purchase a cryo ultramicrotome to prepare cryosections for immunogold electron microscopy.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Research Equipment Initiative 1997 (JE2) [1997]
Funding Scheme	X – not Funded via a specific Funding Scheme

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