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Determination of protein structure by means of MALDI/TOF mass spectrometry

Reference	JEI09401
Principal Investigator / Supervisor	Professor H Dalton
Co-Investigators / Co-Supervisors	Professor Keith Jennings
Institution	University of Warwick
Department	Biological Sciences
Funding type	Research
Value (£)	69,975
Status	Completed
Type	Research Grant
Start date	05/05/1998
End date	05/11/1998
Duration	6 months

Abstract

Funds are requested to assist with the purchase of a matrix-assisted laser desorption ionisation/reflectron time-of-flight (MALDI/TOF) mass spectrometry instrument. The acquisition of the spectrometer will serve primarily to assist in the determination of the relative molecular masses of proteins that are generated on a wide variety of projects concerned with the determination of structure/function relationships. We have identified a number of projects which would benefit immediately including the mechanism of action of anti-viral antibodies, the characterisation of microperoxidases, the interaction of plasminogen-activating proteins to form plasmin and the structural characterisation of side-directed mutants of methane monooxygenase. In addition to these we have identified other projects which are currently under investigation in the Department which range from viral to mammalian proteins. The MALDI/TOF instrument is needed because of its ability to measure biological samples with the minimum amount of clean up and to act as a complementary instrument to the Quattro ESI spectrometer which is currently being used to address many of the problems that can be easily answered by a simpler instrument.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Research Equipment Initiative 1997 (JE2) [1997]
Funding Scheme	X – not Funded via a specific Funding Scheme

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