Award details

A - Automated DNA sequencer for the analysis of gene structure, function and evolution

Reference	JEI09399
Principal Investigator / Supervisor	Dr Robert Old
Co-Investigators / Co-Supervisors	Professor John Davey, Professor Christopher Dowson, Professor Elizabeth Wellington
Institution	University of Warwick
Department	Biological Sciences
Funding type	Research
Value (£)	42,676
Status	Completed
Type	Research Grant
Start date	13/05/1998
End date	13/11/1998
Duration	6 months

Abstract

We propose to establish a DNA sequence analysis facility for the discovery and analysis of signalling molecules active in vertebrate early embryonic development, and active in yeast cell communication. The facility will also underpin studies of antibiotic production by soil bacteria, antibiotic resistance in Streptococcus pneumoniae, and variation in protein virulence determinants in S. pneumoniae. The studies in vertebrate development (by R .W. Old) will be pursued using differential display and novel functional assays to isolate molecules active in patterning gene expression in Xenopus embryo. These assays are designed to lead to novel signalling molecules, to known molecules with novel roles, or in some cases to components of signal transduction pathways, that affect the pattern of expression of key markers of regional specification within the developing embryo. The yeast work (by J. Davey) is centred on pheromone communication in fission yeast, particularly upon how the production of pheromone is regulated and how the response to pheromone is propagated intracellularly. The bacterial work (by C. G. Dowson) includes molecular analysis of beta lactam resistance in Streptococcus pneumoniae by DNA sequence analysis of resistant mutants. Further work on the allelic variation among protein virulence determinants is proposed. A fourth component (by E. H. M. Wellington) is focused on the role of antibiotic production by actinomycete and other antibiotic-producing bacteria in soil. This broad programme, analysing gene function in vertebrate, yeast and bacterial systems, addresses fundamental problems in biology of biotechnological relevance.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Research Equipment Initiative 1997 (JE2) [1997]
Funding Scheme	X – not Funded via a specific Funding Scheme

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