Award details

Metabolic and structural studies of biomolecules by combined chromatography/tandem mass spectrometry

Reference	JEI09392
Principal Investigator / Supervisor	Dr Fred Mellon
Co-Investigators / Co-Supervisors
Institution	Quadram Institute Bioscience
Department	Quadram Institute Bioscience Department
Funding type	Research
Value (£)	146,656
Status	Completed
Type	Research Grant
Start date	30/06/1998
End date	30/12/1998
Duration	6 months

Abstract

New science programmes at IFR require the implementation and development of modern organic mass spectrometric techniques. LC/MS/MS and GC/MS/MS are essential in the metabolic studies of labelled organic micro- and macronutrients in humans, and for structural analysis in human, food structure, food quality and safety, protein engineering and microbiological sciences. Tandem mass spectrometry, combined with LC/MS and CE/MS will increase the information content spectra generated by soft ionisation techniques and improve the specificity and speed of isotope ratio measurements. The instrument will be used collaboratively in at least fifteen different research areas at IFR, all of which involve innovative science. Prominent examples of these are: absorption and metabolic studies of isotope-labelled organic micronutrients in humans, including folates and carotenoids; studies of lipid metabolism in humans; qualitative and quantitative analysis of bioactive food glycosides; characterisation of complex oligosaccharides in foods; confirmation of point mutations in peptide antibiotics and their precursors; accurate molecular weight determination of recombinant proteins (including pectinases and pectic lyases); and analysis of phenolics in the diet. The instrument will be also available to other laboratories on the Norwich Research Park (NRP). All the NRP biochemistry/biotechnology and chemistry based laboratories have expressed interest in using the instrument.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Research Equipment Initiative 1997 (JE2) [1997]
Funding Scheme	X – not Funded via a specific Funding Scheme

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