Award details

Biochemical and genetic analysis of plant cell walls

Reference	JEI09382
Principal Investigator / Supervisor	Professor Simon Turner
Co-Investigators / Co-Supervisors
Institution	The University of Manchester
Department	Life Sciences
Funding type	Research
Value (£)	28,420
Status	Completed
Type	Research Grant
Start date	01/07/1998
End date	01/01/1999
Duration	6 months

Abstract

A new method has successfully been developed using the model plant Arabidopsis thaliana to find novel mutants in plant cell wall formation and vascular tissue development. Following an initial pre-screen the phenotype of mutant plants is characterised by examining stem sections under the light microscope. I now seek to extend this work by isolating new mutants, further characterising the nature of the defect in the mutant plants and to isolate the defective genes. This proposal seeks to facilitate this work by the acquisition of three pieces of equipment. A compound photomicroscope with both fluorescence and DIC optics is required for screening of new mutants as well as histochemical analysis of cell wall and cytoskeletal components. A gas chromatograph is required for detailed biochemical analysis of the phenotype. A video camera is required to record the gels of the many PCR-based genetic markers used in map-based cloning of genes in Arabidopsis.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Research Equipment Initiative 1997 (JE2) [1997]
Funding Scheme	X – not Funded via a specific Funding Scheme

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