Award details

Studies of biomolecular interactions by surface plasmon resonance

Reference	JEI09376
Principal Investigator / Supervisor	Professor Richard Perham
Co-Investigators / Co-Supervisors	Professor Dame Jean Olwen Thomas, Professor Dudley Williams
Institution	University of Cambridge
Department	Biochemistry
Funding type	Research
Value (£)	79,488
Status	Completed
Type	Research Grant
Start date	19/07/1998
End date	19/01/1999
Duration	6 months

Abstract

There is a crucial and pressing requirement in the inter-departmental Cambridge Centre for Molecular Recognition for an instrument to measure the kinetic and thermodynamic parameters of the interactions between macromolecules, and between macromolecules and ligands, in solution. We need to characterise the interaction of proteins in the assembly of specific quaternary strutures and in growth factors and receptors, to analyse the interaction of proteins and nucleic acids in nucleoprotein complexes and signalling pathways, and to characterise the interactions of macromolecules with low molecular mass compounds, such as allosteric effectors, signalling molecules and antibiotics. We have tested the various possible instruments and selected the BIAcore 2000 instrument manufactured by Pharmacia, chiefly because of its versatility, large throughput, and the ability accurately to measure interactions with low molecular mass ligands. We have negotiated a research sponsorship with BIAcore AB to study the development of the instrument in three main areas of interest to ourselves and to the company: phase display, supported lipid bilayers, and DNA-protein interactions. In return for this, BIAcore AB have agreed to pay the University of Cambridge a total sum of 79,490, in the form of a 50 percent price reduction of a BIAcore 2000 instrument system.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Research Equipment Initiative 1997 (JE2) [1997]
Funding Scheme	X – not Funded via a specific Funding Scheme

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