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Award details
Rapid quantitation of analytes from complex biological matrices
Reference
JEI09367
Principal Investigator / Supervisor
Professor Andrew Taylor
Co-Investigators /
Co-Supervisors
Professor Robert Webb
,
Dr Charles Wright
Institution
University of Nottingham
Department
Sch of Biosciences
Funding type
Research
Value (£)
72,032
Status
Completed
Type
Research Grant
Start date
01/01/1998
End date
01/07/1998
Duration
6 months
Abstract
A generic need for LC-MS analysis with high throughput has been identified in three different research areas at the Faculty of Agricultural and Food Sciences. The workers involved have agreed to collaborate in providing a central analytical facility that will draw on the analytical and MS expertise of the Flavour group. Although each group has different analytical problems, they all involve quantification of analytes from complex biological matrices (saliva, blood, plant tissue) in individual samples, not in bulk, representative samples. For instance, the relationship between flavour perception and the amount of a taste compound in saliva has to be determined in an individual person as perception is a highly individualistic response. In farm animals, reproduction is crucial to economic success and the chemical factors influencing ovulation need to be elucidated, not generally, but in target tissues of each animal, to understand the mechanisms governing ovulation. In tomatoes and pork chops, the quality is judged by consumers, not on an average basis but on the actual fruit or chop consumed; therefore it is essential to know the range of variation for the chemicals responsible for quality in these commodities, so as to provide an objective measure of food quality. At the heart of the analytical solution is a Micromass API-MS which is capable of detecting and quantifying molecules ranging from 30Da to 100kDa in the liquid or gas phases. This, coupled with the state of the art Z spray interface developed by Micromass, provides an extremely flexible system, designed to cope with analysis of molecules from complex biological matrices. The applicants have successful track records in research and the Food Science and Animal Science groups were rated 5 star in the last RAE exercise. The request is for 72,034 or 47 percent of the purchase price of the equipment.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Joint Research Equipment Initiative 1997 (JE2) [1997]
Funding Scheme
X – not Funded via a specific Funding Scheme
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