Award details

Rapid quantitation of analytes from complex biological matrices

ReferenceJEI09367
Principal Investigator / Supervisor Professor Andrew Taylor
Co-Investigators /
Co-Supervisors
Professor Robert Webb, Dr Charles Wright
Institution University of Nottingham
DepartmentSch of Biosciences
Funding typeResearch
Value (£) 72,032
StatusCompleted
TypeResearch Grant
Start date 01/01/1998
End date 01/07/1998
Duration6 months

Abstract

A generic need for LC-MS analysis with high throughput has been identified in three different research areas at the Faculty of Agricultural and Food Sciences. The workers involved have agreed to collaborate in providing a central analytical facility that will draw on the analytical and MS expertise of the Flavour group. Although each group has different analytical problems, they all involve quantification of analytes from complex biological matrices (saliva, blood, plant tissue) in individual samples, not in bulk, representative samples. For instance, the relationship between flavour perception and the amount of a taste compound in saliva has to be determined in an individual person as perception is a highly individualistic response. In farm animals, reproduction is crucial to economic success and the chemical factors influencing ovulation need to be elucidated, not generally, but in target tissues of each animal, to understand the mechanisms governing ovulation. In tomatoes and pork chops, the quality is judged by consumers, not on an average basis but on the actual fruit or chop consumed; therefore it is essential to know the range of variation for the chemicals responsible for quality in these commodities, so as to provide an objective measure of food quality. At the heart of the analytical solution is a Micromass API-MS which is capable of detecting and quantifying molecules ranging from 30Da to 100kDa in the liquid or gas phases. This, coupled with the state of the art Z spray interface developed by Micromass, provides an extremely flexible system, designed to cope with analysis of molecules from complex biological matrices. The applicants have successful track records in research and the Food Science and Animal Science groups were rated 5 star in the last RAE exercise. The request is for 72,034 or 47 percent of the purchase price of the equipment.

Summary

unavailable
Committee Closed Committee - Agri-food (AF)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative Joint Research Equipment Initiative 1997 (JE2) [1997]
Funding SchemeX – not Funded via a specific Funding Scheme
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