Award details

Imaging plant signal transduction

Reference	JEI09358
Principal Investigator / Supervisor	Professor Marc Knight
Co-Investigators / Co-Supervisors	Professor Malcolm Campbell, Professor Mark Fricker
Institution	University of Oxford
Department	Plant Sciences
Funding type	Research
Value (£)	34,736
Status	Completed
Type	Research Grant
Start date	19/07/1998
End date	19/01/1999
Duration	6 months

Abstract

This proposal is for research aimed at investigating the dynamic events occurring during the transduction of environmental signals in plants. Calcium signalling will be imaged in intact whole plants responding to different abiotic stresses, by the use of the calcium- activated photoprotein, aequorin. Aequorin will be expressed in transgenic Arabidopsis thaliana. Using a highly-sensitive photon-counting camera (for which the funds are requested) we will be able to measure both spatially, and in real time, calcium signalling in intact whole plants responding to cold, heat, oxidative stress, drought and mechanical stimulation. In this way we will be able to 'map' the contributions of this important signal transduction component, in different tissues and cells. This will allow for the first time an understanding of how the information from these different stresses, received by plants, are then converted into a specific response and also what the commonalities/interactions between these pathways are at the level of calcium signalling. Additionally we will use this imaging approach to screen for calcium signalling mutants of Arabidopsis. This will allow for the first time the identificaton of specific mutations early in abiotic stress transduction cascades.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Research Equipment Initiative 1997 (JE2) [1997]
Funding Scheme	X – not Funded via a specific Funding Scheme

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