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Time resolved infra-red spectroscopy of metallo proteins and related chemistry

Reference	JEI09281
Principal Investigator / Supervisor	Professor R Thorneley
Co-Investigators / Co-Supervisors
Institution	John Innes Centre
Department	Disease and Stress Biology
Funding type	Research
Value (£)	60,428
Status	Completed
Type	Research Grant
Start date	01/08/1998
End date	01/02/1999
Duration	6 months

Abstract

To provide the John Innes Centre, with a unique, anaerobic, multi- mixing, stopped-flow Fourier transform infra-red spectrometer (SF- FTIR) for transient kinetic studies, principally of small molecule binding and activation of metal sites in enzymes and chemical complexes, with the aim of elucidating mechanisms of catalysis at atomic resolution. Nitrogenase and related chemistry; Oxalate oxidase germin; Cu-containing nitrite and N20 reductases and related chemistry; Cyt. bo3, a haem-Cu oxide (with UEA); Cyt.cd1 nitrite reductase (with Oxford) will be studied initially. These are Foresight priorities in catalysis, chemical nitrogen fixation and environment/pollution, denitrification and water purification. The equipment is a development of a prototype instrument built by Dr C. Wharton (Birmingham, Biochemistry) for proteinase and protein folding studies. The feasibility of using S-F FTIR for metallo-protein studies and related chemistry has recently been shown by the applicant at Birmingham (George et al, J. Amer. Chem. Soc. 1997, 119, 6450-6451). The JIC facility will be made available to other groups on the Norwich Research Park, principally members of the BBSRC Centre for Metallo-protein Spectroscopy and Biology at UEA (Directors Prof. A. J. Thomson & Prof. C. Greenwood) and the UK research community in academia (e.g. Prof. S. J. Ferguson, Oxford, Biochemistry) and industry (e.g. Unilever). In addition to Unilever, a financial contribution has been obtained from Hi-Tech Ltd, a UK SME who will develop and supply a new multi- mixing drive and sample handling unit.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Research Equipment Initiative 1997 (JE2) [1997]
Funding Scheme	X – not Funded via a specific Funding Scheme

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