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Award details
Use of DNA sequencing to identify single nucleotide polymorphisms and to analyse testis specific genes and their promotors
Reference
JEI09279
Principal Investigator / Supervisor
Professor Nabeel Affara
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Pathology
Funding type
Research
Value (£)
25,000
Status
Completed
Type
Research Grant
Start date
01/01/1998
End date
01/07/1998
Duration
6 months
Abstract
This project aims to develop three different areas of genome analysis in collaboration with PIC. (1) sequencing porcine clones containing microsatellite markers will be used to identify Single Nucleotide Polymorphisms (SNPs). These will be used to develop a high throughput genetic analysis system by means of oligonucleotide micro-arrays. The aim is to develop 200 SNPs covering the porcine genome. (2) Development of expressed sequence tags (ESTs) from a subtracted human testis cDNA library greatly enriched in testis- specific genes. These ESTs will be used to identify genes with important roles in spermatogenesis and fertility through expression analysis, comparative mapping on the mouse and human genomes to permit correlation to mutations affecting fertility, and complete sequence analysis of promising transcripts. (3) Comparative sequence analysis of testis-specific promoter regions to identify important motifs critical for regulating expression in germ cells. This will involve comparisons between testis-specific genes in the same species and between species (mouse, human and pigs) and will provide the basis for the functional studies to identify motifs regulating germ cell transcripts.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Joint Research Equipment Initiative 1997 (JE2) [1997]
Funding Scheme
X – not Funded via a specific Funding Scheme
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