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Fourier transform infared spectroscopy of electron transfer membrane proteins
Reference
JE616840
Principal Investigator / Supervisor
Professor Peter Rich
Co-Investigators /
Co-Supervisors
Institution
University College London
Department
Genetics Evolution and Environment
Funding type
Research
Value (£)
30,956
Status
Completed
Type
Research Grant
Start date
08/04/2002
End date
08/04/2003
Duration
12 months
Abstract
The machinery will be used for FTIR spectroscopic studies in the 800-3000 cm-1 range of membrane proteins. This frequency range covers vibrational changes of ligands and protein and the aim is to identify specific amino acids and cofactor changes that are of functional significance. Such studies on cytochrome c oxidase are already in progress with a heavily-used FTIR spectrometer funded by the Wellcome Trust. Funds are requested funding for a second machine, dedicated primarily to the ATR method, so that the studies can be extended to other electron transfer enzymes, namely complex I, bc1 complex and thylakoid bf complex, and to other electron transfer proteins in selected collaborations. The second machine will provide much needed user time and, by configuring the machines differently, and will more efficiently allow use of the complementary methods of transmission FTIR using CaF2 windows and ATR (attenuated total reflectance)-FTIR using thin films of membrane proteins that have been deposited on the silicon ATR prism.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Joint Equipment Initiative 2001 (JE6) [2001]
Funding Scheme
X – not Funded via a specific Funding Scheme
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