Award details

High troughput DNA sequencing and microarray analysis in the JIC genome centre

Reference	JE616553
Principal Investigator / Supervisor	Professor Michael Bevan
Co-Investigators / Co-Supervisors	Professor Ian Bancroft
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	200,000
Status	Completed
Type	Research Grant
Start date	01/04/2002
End date	01/04/2003
Duration	12 months

Abstract

The new Genome Centre at the John Innes Centre will be completed at the end of 2001 and will house the Reduction to Practice Laboratory, and the Syngenta Wheat Improvement Centre. The purpose of the Genome Centre is to provide all JIC scientists and collaborators with access to state of the art equipment for high throughput analysis of DNA sequences, for genotyping crop plant crops for mRNA analysis using array technology, and for physical mapping and gene isolation from crop plants. The Genome Centre will house and operate equipment to benefit a wide range of plant and microbial research at the John Innes Centre. One of the central requirements of genome research is DNA sequencing. The development of this technology is characterised by increased accuracy and throughput and reduced unit costs, primarily due to automation of key steps. This proposal aims to establish automated plasmid preparations and sequence reactions, and integrate this with improved sequencing methods. The system will be integrated by a database and sample tracking system. A DNA analyser for microarray analysis is also requested to supplement existing microarray equipment.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Equipment Initiative 2001 (JE6) [2001]
Funding Scheme	X – not Funded via a specific Funding Scheme

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