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Quantification of mature and immature collagen cross-links in engineered and natural tissues

Reference	JE616492
Principal Investigator / Supervisor	Professor Anthony Hollander
Co-Investigators / Co-Supervisors	Dr Michael Billingham, Dr Mark Perry, Mr Trevor Sims, Professor John Tarlton, Professor Jonathan Tobias
Institution	University of Bristol
Department	Department of Academic Rheumatology
Funding type	Research
Value (£)	28,756
Status	Completed
Type	Research Grant
Start date	01/01/2002
End date	01/01/2003
Duration	12 months

Abstract

There are three objectives of the work to be undertaken with the new equipment: 1). To determine the relationship between crosslink maturity and tensile strength in engineered cartilage. Cartilage will be engineered from bovine or human chondrocytes seeded onto biodegradable scaffolds. The engineered tissue will be subjected to tensile load to failure testing on an Instron 6220. The concentration of mature and immature collagen crosslinks will be measured in the engineered tissues using the new amino acid analyser and related to tensile strength. Preliminary data indicates that the engineered tissues will contain sufficient crosslinks for detection by this method; 2). To monitor the effects of non-enzymic glycation cross-linking on tensile strength. Cartilage will be engineered with or without subsequent exposure to ribose. The concentration of resulting glycation crosslinks will be monitored using the new amino acid analyser and tensile strength will be measured as above. In this way we will determine if the tensile strength of engineered tissues can be enhanced by controlled glycation; and 3). To measure collagen cross-links in series of independent studies. Crosslinks will be measured in a variety of normal and pathological tissues in a series of independent studies by different connective tissue biologists in Bristol. In this way the new equipment will provide an important resource for on-going multidisciplinary connective tissue research as well as for the tissue engineering projects described above.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Equipment Initiative 2001 (JE6) [2001]
Funding Scheme	X – not Funded via a specific Funding Scheme

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