Award details

Metabolic profiling of sugars sugar phosphates nucleotide sugars and antioxidants in plants and fungi

Reference	JE616481
Principal Investigator / Supervisor	Professor Nicholas Smirnoff
Co-Investigators / Co-Supervisors	Professor Nicholas Talbot
Institution	University of Exeter
Department	Biosciences
Funding type	Research
Value (£)	28,088
Status	Completed
Type	Research Grant
Start date	30/06/2002
End date	30/06/2003
Duration	12 months

Abstract

The LC-EC system will be used for analysing sugars, sugar phosphates and nucleotide sugars with high sensitivity and selectivity by pulsed amperometric detection. It also allows detection of antioxidants (including ascorbate) in DC amperometric mode. These analyses are relevant to the following projects. 1. Developing metabolic profiling methodology for carbohydrates and antioxidants. Methods of wide applicability for profiling carbohydrates and antioxidants in plants and fungi will be developed and evaluated. 2. Engineering mannose and ascorbate metabolism in plants. Understanding the control of these related pathways in mutants and transgenic plants requires measurement of the phosphorylated sugar intermediates and ascorbate. 3. The role of sugars in the pathogenicity of rice blast fungus. Glycerol, trehalose and arabitol are involved in the pathogenicity of rice blast. The function and metabolism of these sugars will be investigated in knockout mutants. 4. The function of erythroascorbate in rice blast. The synthesis and function of erythroascorbate, an ascorbate analogue found in fungi, will be investigated.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Equipment Initiative 2001 (JE6) [2001]
Funding Scheme	X – not Funded via a specific Funding Scheme

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