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Gene tranfer studies using adenoviral vectors

Reference	JE616459
Principal Investigator / Supervisor	Professor Sergey Kasparov
Co-Investigators / Co-Supervisors	Dr Julian Paton, Dr Anja Gabriele Teschemacher
Institution	University of Bristol
Department	Physiology and Pharmacology
Funding type	Research
Value (£)	65,736
Status	Completed
Type	Research Grant
Start date	17/05/2002
End date	17/05/2003
Duration	12 months

Abstract

Somatic gene transfer using replication deficient adenoviral vectors is a powerful tool to determine gene function. We wish to employ this technology to study neuronal mechanisms of signal transduction both in vivo and in vitro. Adenoviral vectors allow an effective means of transgene expression, which can be (i) localised within brain, (ii) targeted to a particular cellular type and (iii) controlled by drugs. These features make this approach unique for physiological analysis. Although we already use adenoviral vectors, there is no facility within our departments where they can be engineered and proliferated. We wish to equip a new molecular biology laboratory in the Integrated Cell Signalling Laboratories space created in the School of Medical Sciences using the Wellcome Trust JIF award. Our current scientific enquiries demand: (i) selective inactivation of endothelial nitric oxide synthase (eNOS) in brainstem nuclei controlling arterial pressure; (ii) the study of mechanisms of nitric oxide production within specific brainstem regions; (iii) the measurement of intracellular Ca2+ and cyclic GMP in neurones using genetically engineered indicator proteins; (iv) the study of the intracellular cascades activated by nitric oxide in GABAergic neurones. These studies will reveal novel mechanisms of sensory integration relevant for central control of arterial pressure.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Equipment Initiative 2001 (JE6) [2001]
Funding Scheme	X – not Funded via a specific Funding Scheme

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