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Fermentation in post-genomic physiology studies and biomass production for investigating protein function

Reference	JE616455
Principal Investigator / Supervisor	Dr Diane Kelly
Co-Investigators / Co-Supervisors	Professor John Barrett, Professor Peter Brophy, Professor Douglas Kell, Professor Steven Kelly, Dr Ross King, Professor David Lamb, Professor Michael Young
Institution	Aberystwyth University
Department	Inst of Biological, Environ & Rural Sci
Funding type	Research
Value (£)	165,040
Status	Completed
Type	Research Grant
Start date	01/01/2002
End date	01/01/2003
Duration	12 months

Abstract

Fermentation vessels are central to accurate control of growth conditions. Continuous culture and turbidistat studies provides steady state conditions desirable for reproducible and quantitative investigations for transcriptome and proteome analysis. Other classical physiology experiments also require modern fermenter technology. Efficient scale-up of heterologous protein production for structure function studies in parallel for volumes of greater than 10L, is facilitated by such equipment rather than flask cultures using orbital shakers. The equipment requested will allow the flexible use of modern fermentation technology in BBSRC research projects so that different users can utilise these at any one time. It will allow investigation of transcriptome and proteome studies in yeast, pathogenic fungi and mycobacteria, on the cytochromes P450 superfamily of Streptomyces coelicolor, C. elegans glutathione transferases, bacterial cytokines, microbial and mammalian biotransformations, and the production of recombinant protein in yeast and Escherichia coli.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Equipment Initiative 2001 (JE6) [2001]
Funding Scheme	X – not Funded via a specific Funding Scheme

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