BBSRC Portfolio Analyser
Award details
Implementation of a novel real-time confocal microscope for studying dynamic subcellular processes
Reference
JE616454
Principal Investigator / Supervisor
Dr Peter Lipp
Co-Investigators /
Co-Supervisors
Dr Martin Bootman
Institution
Babraham Institute
Department
Molecular Signalling
Funding type
Research
Value (£)
93,600
Status
Completed
Type
Research Grant
Start date
29/01/2002
End date
29/01/2003
Duration
12 months
Abstract
This award will support a novel ultra-fast confocal microscope based on a variable-speed Nipkow disk. The essential new technology is a scan head with a Nipkow disk that can rotate at speeds up to 1000 Hz. The variable speed Yokogawa scan head will present imagers a new technical edge in the ability to monitor rapid dynamic biological processes in living cells. In combination with a Hammatsu cooled intensifier and a CCD camera minimal excitation energies can be used, thus imparting to the Yokogawa system the significant advantage of intrinsically low sample bleaching. The spatial resolution of the existing Yokogawa system is well known to be excellent, but it has lacked speed. Therefore, the new system will represent the best of both worlds: fast frame acquisition and high-resolution images. This is ideal for studies of dynamic subcellular signalling events such as cardiac excitation-contraction coupling and protein translocation.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Joint Equipment Initiative 2001 (JE6) [2001]
Funding Scheme
X – not Funded via a specific Funding Scheme
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