Award details

Characterising molecular interactions using real-time optical biosensor approaches

Reference	JE616418
Principal Investigator / Supervisor	Professor Jeremy Turnbull
Co-Investigators / Co-Supervisors	Professor Rudolf Allemann, Dr Stephen Dove, Professor Chris Franklin, Dr Eva Hyde, Dr Barry Levine, Professor Peter Lund, Professor Christopher Thomas, Dr Rosemary Waring
Institution	University of Birmingham
Department	Sch of Geography, Earth & Env Sciences
Funding type	Research
Value (£)	66,300
Status	Completed
Type	Research Grant
Start date	01/03/2002
End date	01/03/2003
Duration	12 months

Abstract

Studies on protein-protein and protein-ligand interactions underpin the detailed understanding of fundamental biological processes. Optical biosensors permit study of molecular recognition events between various binding partners (from small molecules to cells) in real-time, at low concentrations and without labelling. Binding partners can be rapidly identified and parameters such as affinity, kinetics (on and off rates), and concentration readily measured. The aim of this proposal is to purchase a state-of-the-art waveguide optical biosensor instrument to be applied to characterisation of molecular interactions in a range of biological problems. We describe two general themes for which the acquisition of this instrument will play a key enabling role. The first is protein-protein interactions. Examples include transcription factors, plant self-incompatibility proteins, bacterial chaperones and muscle contractile proteins. The second general theme covers other protein-ligand interactions, including interactions of proteins with DNA, complex sugars, phospholipids and nucleotides. Funding for this instrument will also provide the opportunity for an active collaboration with the instrument manufacturer to develop novel and improved biosensor applications. This technology will be a valuable addition to the research infrastructure available in Birmingham for studying the basis of molecular interactions.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Equipment Initiative 2001 (JE6) [2001]
Funding Scheme	X – not Funded via a specific Funding Scheme

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