Award details

(A) FACS/flow cytometry: advanced applications in microbiology and immunology

Reference	JE514686
Principal Investigator / Supervisor	Professor Elizabeth Wellington
Co-Investigators / Co-Supervisors	Professor Clive Brasier, Professor David Scanlan
Institution	University of Warwick
Department	Biological Sciences
Funding type	Research
Value (£)	60,000
Status	Completed
Type	Research Grant
Start date	26/09/2001
End date	26/03/2002
Duration	6 months

Abstract

Fluorescence activated cell sorting (FACS)/flow cytometry is a technique for identifying and sorting cells on the basis of light emitted from fluorochromes upon excitation with monochromatic light or scattered by the cell. Our proposal is to extend the technique to the study of bacterial physiology in complex ecological niches and also for advanced techniques in transplant immunology. We will use FACS to analyse the interaction between pathogenic bacteria and protozoa, particularly how bacteria may survive within protozoa. There is evidence suggesting that this provides a means of protecting the bacteria against biocides thus is of potential importance in water borne diseases such as legionellosis. Additionally we will use FACS to analyse bacteria in complex mixtures, particularly of marine picoplankton, identifying cells by a suite of genotyping methods, characterising the metabolic state of the cells using fluors to tag cells internally via metabolism of labelled substrates. We will monitor the biodiversity and distribution of the heterotrophic component, identifying 'consortia' of microorganisms with defined distribution and potentially sorting such organisms to bring into culture such as consortia and from this ultimately dissecting the function of individual species. Finally, we will use FACS in combination with peptide-HLA tetramer reagents to identify and characterise cytotoxic T lymphocytes responding to human minor histocompatibility antigens.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Equipment Initiative 2000 (JE5) [2000]
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search