Award details

(A) NMR spectrometer enhancements for applications of structural analysis to high molecular weight proteins

Reference	JE514382
Principal Investigator / Supervisor	Dr Paul Driscoll
Co-Investigators / Co-Supervisors	Dr Christopher Fletcher, Professor John Ladbury, Dr Mark Pfuhl
Institution	University College London
Department	Structural Molecular Biology
Funding type	Research
Value (£)	74,360
Status	Completed
Type	Research Grant
Start date	01/01/2002
End date	01/07/2002
Duration	6 months

Abstract

The application is for the purchase of hardware to add extra capabilities to an existing 600 MHz NMR spectrometer, specifically the provision of a fourth radiofrequency (RF) channel for use with deuterium (2H,D) labelled proteins, and the updating of the spectrometer console to incorporate digital signal handling and other new functionality. These items will allow us to employ the state-of-the-art in NMR spectroscopic techniques to probe the solution 3D structure, dynamic and functional properties of many biological macromolecules that are too large (i.e. Mr > 20,000) to be studied in detail using the present spectrometer configuration. In this application we provide a case for funding of the proposed upgrade which will open new cost-effectiveness avenues to determine the structure-function properties of a range of proteins under investigation by four separate research groups in the department. These projects include the analysis of protein structure, dynamics, thermodynamics, solvation and ligand-binding functions in a variety of different biochemical contexts ranging from tyrosine kinase signal transduction. NO/arginine metabolism, bacterial nucleoid structure, muscle physiology, apoptosis, phospholipid kinase regulation, mammalian chaperonins, and the molecular pathology of hepatitis C.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Equipment Initiative 2000 (JE5) [2000]
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search