Award details

(A) A multi-detection microplate reader for the rapid analysis of disease processes and biomaterials

Reference	JE514370
Principal Investigator / Supervisor	Professor A Brook
Co-Investigators / Co-Supervisors	Professor Fiona Boissonade, Professor Ian Douglas, Dr Gareth Evans, Dr Yue Guan, Dr B Stringer
Institution	University of Sheffield
Department	University of Sheffield Department
Funding type	Research
Value (£)	14,974
Status	Completed
Type	Research Grant
Start date	23/03/2001
End date	23/09/2001
Duration	6 months

Abstract

Cellular properties associated with disease processes can be characterised by various molecular markers in the cell or the coupled metabolic fluxes. Innovations in biomaterials for bone and tooth replacement require rapid assessment of the surface properties of the materials and their effect on the host system. Clinically, it is important to make measurements based on small sample volumes. Many of these measurements can be made using a single instrument, a BMG POLARstar multi-detection microplate reader. For the projects outlined, the use suitable fluorescence and luminescence tagging systems will enable this instrument to quantify both early and late inflammation signals, to assess the influence of some exogenous factors on the development of inflammation, and to sensitively detect neuronal excitability regulators reflecting neuropathic and inflammatory changes. The microplate reader will be intensively employed for screening adherent and anti-adherent molecules by both fluorescence and absorbance. A further application is to characterise the surface functionality of conventional biomaterials and some properties of metabolisable biomaterials. In addition, this instrument can contribute to establishing a rapid detection system for quantifying oral microbial cells and the present work investigating the link between oral bacteria and infective endocarditis.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Equipment Initiative 2000 (JE5) [2000]
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search