Award details

(A) A quadrupole/time of flight mass spectrometer for functional proteomics and the analysis of postranslational protein modifications

Reference	JE514343
Principal Investigator / Supervisor	Professor Walter Kolch
Co-Investigators / Co-Supervisors	Professor John Coggins, Professor Andrew Pitt
Institution	University of Glasgow
Department	IBLS Division of Biochemistry & Molecula
Funding type	Research
Value (£)	147,008
Status	Completed
Type	Research Grant
Start date	11/09/2001
End date	11/03/2002
Duration	6 months

Abstract

This application is for funds to purchase a Q-STAR quadrupole/time of flight mass spectrometer for the MS/MS analysis of protein samples of biological importance. MS/MS will allow us to determine peptide sequences and to map the sites of posttranslational modifications. The combination of high sensitivity with high resolution and mass accuracy makes this instrument particularly useful for proteomics studies, where typically only minute amounts of protein are available for analysis. The Q-STAR will be used for 1) sequencing proteins of interest in order to determine of verify identity; 2) mapping of posttranslational modifications in proteins; 3) characterisation of protein signalling complexes using a proteomics approach; and 4) identification of differentially expressed proteins in conjunction with 2D gel expression profiling. The Q-Star will complement the MALDI-TOF instrument (Perkin Elmer Voyager DE PRO) which we have recently purchased as the first stage of establishing a Proteomics Facility in the Institute of Biomedical & Life Sciences at Glasgow University. The strength of the MALDI-TOF (peptide mass fingerprinting) superbly synergises with the strength of the Q-STAR (peptide sequencing and mapping of posttranslational modifications). The proteomics facility will be accessible to researchers from the Universities of Glasgow and Strathclyde and the Beatson Institute.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Equipment Initiative 2000 (JE5) [2000]
Funding Scheme	X – not Funded via a specific Funding Scheme

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