Award details

(A) Gene detection and expression analysis in aquaculture research

Reference	JE514328
Principal Investigator / Supervisor	Professor Alan Teale
Co-Investigators / Co-Supervisors	Professor Sandra Adams, Professor stephen george
Institution	University of Stirling
Department	Institute of Aquaculture
Funding type	Research
Value (£)	20,000
Status	Completed
Type	Research Grant
Start date	01/04/2001
End date	01/10/2001
Duration	6 months

Abstract

Real time PCR offers a number of advantages over conventional PCR, including increased speed and lower costs in accessing DNA amplification data. This is a particularly valuable feature in a diagnostic setting. For purposes of gene transcript quantification in studies of gene expression, the ability to examine the kinetics of amplification provides greater accuracy in measurements of target transcript numbers than is possible with conventional PCR. We propose to aim to obtain a real time PCR system that is capable of handling internal controls in individual reactions for use in our laboratories. This will be used to a) develop and apply new, rapid diagnostic tests capable of detecting important fish pathogens, and b) study gene expression in three forms of adaptation (i) dietary adaptation in fish; (ii) adaptation to pollutant challenge in fish; and (iii) adaptation of salmon lice to the host during the transition from free-living to host-settled stages.

Summary

unavailable

Committee	Closed Committee - Animal Sciences (AS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Equipment Initiative 2000 (JE5) [2000]
Funding Scheme	X – not Funded via a specific Funding Scheme

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