Award details

(A) Large scale analysis of gene expression using microarray technology and quantitative PCR

Reference	JE514314
Principal Investigator / Supervisor	Dr Roger Watson
Co-Investigators / Co-Supervisors	Professor Martin Allday, Professor Geoffrey Smith
Institution	Imperial College London
Department	Dept of Medicine
Funding type	Research
Value (£)	64,917
Status	Completed
Type	Research Grant
Start date	30/08/2001
End date	28/02/2002
Duration	6 months

Abstract

The purpose of this application is to enable us to quantitatively analyse gene expression in the cell cycle, cancer and virus systems which we are studying. The Ludwig Institute is well advanced in implementing a DNA microarray programme through a joint venture with the ICRF and the Wellcome Trust. This microarray programme will produce arrays of human and mouse genes at the Sanger Centre and supply the arrayed chips to our unit for analysis. Supply of the chips will start in July 2000 and the agreement will run for 5 years in the first instance. The use of the chips will be available to Ludwig Institute staff and associated Imperial College staff within the Virology and Cell Biology Section of the Department of Infectious Diseases. The analysis in the planned facility requires us first to identify genes of interest from the microarrays and then to be able to quantitate the levels of their mRNAs in cell and tissue samples. The major equipment items required for the facility are the array scanner and associated computer plus a real time quantitative PCR machine which will allow quantitative of the mRNAs of interest.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Equipment Initiative 2000 (JE5) [2000]
Funding Scheme	X – not Funded via a specific Funding Scheme

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