Award details

(A) In vivo fluorescence microscopy

Reference	JE514278
Principal Investigator / Supervisor	Professor J East
Co-Investigators / Co-Supervisors	Professor Karen Lillycrop, Dr G Sale
Institution	University of Southampton
Department	Centre for Biological Sciences
Funding type	Research
Value (£)	40,316
Status	Completed
Type	Research Grant
Start date	01/06/2001
End date	01/12/2001
Duration	6 months

Abstract

The use of chimeric molecules containing green fluorescent protein has, for the first time, allowed us to visualise, using fluorescence microscopy, a wide range of intracellular processes in vivo and in real time. It is clear that this powerful approach for examining cellular processes is set to expand and in order to make full use of this technique, a dedicated inverted stage epifluorescence microscope facility is required. Although all the individual pieces of apparatus are available within the School of Biological Sciences, they are tied to other projects and in addition to availability, there are compatibility problems. In order that the expression and monitoring of GFP chimeras can be effectively carried out, it is essential that the microscope be fully equipped so that transfection and monitoring of fluorescent chimeras can be undertaken on the same set up. The projects range from the pathogenesis of microbiological organisms to cell signalling and cell targeting. It is envisaged that this will be a School-wide facility which will encourage the development of real-time imaging of cellular functions and serve to enhance and widen our research output.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Equipment Initiative 2000 (JE5) [2000]
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search