Award details

(A) A time-resolved CD spectrophotometer for protein biochemistry at UEA

Reference	JE412716
Principal Investigator / Supervisor	Dr Nicholas James Watmough
Co-Investigators / Co-Supervisors	Professor Colin Kleanthous, Dr S Spiro
Institution	University of East Anglia
Department	Biological Sciences
Funding type	Research
Value (£)	55,804
Status	Completed
Type	Research Grant
Start date	28/01/2000
End date	28/07/2000
Duration	6 months

Abstract

Request is made for a contribution towards the purchase of a novel CD-spectrometer capable of both steady-state and time-resolved measurements. The instrument will be used to support a number of ongoing projects in Bacterial Physiology and Biochemistry Group based in the School of Biological Sciences at UEA. These projects encompass three main themes: assessing protein conformation, protein:protein interactions and redox-linked conformational changes. The principal requirement is for far-UV CD spectroscopy but there is also a need to use CD to examine the environment of some co- factors such as flavins. A particular feature of this instrument is the ability to make absorbance and CD measurements on the same sample. This is essential for a number of projects where we seek to temporarily relate changes in the redox- or ligation-state of a chromophore to conformational change. In addition we have clearly identified a wider user community at UEA and on the nearby Norwich Research Park who will be able to use this instrument to support work in the areas of metalloproteinase biochemistry, plant disease resistance and structural biology.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Equipment Initiative 1999 (JE4) [1999]
Funding Scheme	X – not Funded via a specific Funding Scheme

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