Award details

(A) Trace analysis using capillary electrophoresis and capillary electrochromatography coupled with mass spectrometry

Reference	JE412714
Principal Investigator / Supervisor	Professor Simon James Gaskell
Co-Investigators / Co-Supervisors	Professor Robert Beynon
Institution	The University of Manchester
Department	Chemistry
Funding type	Research
Value (£)	19,428
Status	Completed
Type	Research Grant
Start date	26/01/2000
End date	26/07/2000
Duration	6 months

Abstract

This proposal requests funds for the purchase of capillary electrophoresis instrumentation in support of multiple inter-departmental and inter-institutional collaborative research projects. The projects share a common requirement for unequivocal identification of trace components of mixtures and this will be addressed by the coupling of electrophoresis (including isotachophoresis and isoelectric focusing) or electrochromatography with state-of-the-art mass spectrometry and tandem mass spectrometry. The research to benefit from the new instrumentation is largely concerned with the characterisation of molecules of biological importance, though we have also already demonstrated the utility of capillary electrophoresis-tandem mass spectrometry in the characterisation of textile dyestuffs. In the area of protein and peptide analyses we will apply the new instrumentation in proteomics research, in the study of immunologically important peptides, in the characterisation of major urinary proteins in the mouse, and in the elucidation of structural changes accompanying the oxidation of low-density lipoprotein. Applications to the analysis of small molecules will include the determination of a vitamin B6 metabolite, 4-pyridoxic acid, and the characterisation of the yeast metabolome.

Summary

unavailable

Committee	Closed Committee - Biomolecular Sciences (BMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Equipment Initiative 1999 (JE4) [1999]
Funding Scheme	X – not Funded via a specific Funding Scheme

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