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(B) Q-Tof electrospray mass spectrometry and proteome analysis of important aspects of microbes and plants

Reference	JE412701
Principal Investigator / Supervisor	Professor Keith Chater
Co-Investigators / Co-Supervisors	Dr Fred Mellon, Dr Michael Naldrett
Institution	John Innes Centre
Department	Cell and Develop Biology
Funding type	Research
Value (£)	289,408
Status	Completed
Type	Research Grant
Start date	01/03/2000
End date	01/09/2000
Duration	6 months

Abstract

Q-ToF ES MS will be used to sequence modified and unmodified proteins after partial purification (often by 2D electrophoresis) from microbial cultures, plant tissues, or sub- cellular fractions, in order to relate proteins to genes sequenced in genome projects (Streptomyces, E. coli, Arabidopsis, rice, wheat) or to permit oligonucleotides to be designed for gene searches in unsequenced organisms (Azotobacter, Rhizobium, pea, brassicas, other cereals). Q-ToF ES MS will also be used to examine changes in protein abundance or modification during development, exposure to stress, and production of valuable products. The high sequencing ability, but comparatively low throughput, of the Q-ToF MS will complement the high throughput mass fingerprinting of a MALDI-ToF MS which is about to be installed at JIC. The use of the Q-ToF MS will be particularly valuable in capitalising on ongoing JIC-led genome sequence determinations for two major model organisms, Streptomyces coelicolor A3(2) and Arabidopsis thaliana.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Equipment Initiative 1999 (JE4) [1999]
Funding Scheme	X – not Funded via a specific Funding Scheme

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