Award details

(A) Development of an integrated platform for rapid protein identification and characterisation

Reference	JE412695
Principal Investigator / Supervisor	Professor Stephen Pennington
Co-Investigators / Co-Supervisors	Professor Huw Rees
Institution	University of Liverpool
Department	Human Anatomy and Cell Biology
Funding type	Research
Value (£)	135,000
Status	Completed
Type	Research Grant
Start date	22/11/2000
End date	22/05/2001
Duration	6 months

Abstract

Dramatic progress has been made in genome sequencing and functional genomics. However, it has become apparent that investigation of the growing number of genes of unknown function additionally requires systematic approaches to protein identification, characterisation and protein-based methods for functional analysis. Peptide mass fingerprinting by Matrix Assisted Laser Desorption Ionisation (MALDI) mass spectrometry potentially provides a rapid and high-throughput method for identification and characterisation of proteins. The power of this approach to protein identification will increase as DNA and protein sequence databases mature and with improvements in post source decay (PSD) analysis. The primary objective of this proposal is to integrate the use of three items of instrumentation: automated protein digestion robot, and automated protein digest 'clean up' and sample target loading machine and a new state-of-the-art dual reflectron MALDI mass spectrometer. This will provide a platform for rapid and potentially high-throughput protein characterisation to meet the needs of research programmes in the applicant's laboratories and of the significant grouping of biomedical researchers in Liverpool.

Summary

unavailable

Committee	Closed Committee - Engineering & Biological Systems (EBS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Equipment Initiative 1999 (JE4) [1999]
Funding Scheme	X – not Funded via a specific Funding Scheme

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