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(A) High throughput screening and analysis in combinatorial chemistry
Reference
JE412671
Principal Investigator / Supervisor
Professor Mark Bradley
Co-Investigators /
Co-Supervisors
Institution
University of Southampton
Department
Sch of Chemistry
Funding type
Research
Value (£)
113,632
Status
Completed
Type
Research Grant
Start date
31/10/2000
End date
30/04/2001
Duration
6 months
Abstract
Mass spectrometry has proved to be highly suitable for combinatorial applications due to its sensitivity, analysis speed, automation capability and compatibility with solution phase techniques. We propose to expand the use of HPLC-MS in the combinatorial area developing two novel screening methods. Firstly, using a process termed 'pulsed' ultrafiltration in which enzyme and library components are dialysed prior to MS analysis. Secondly, analysing complete assay mixtures containing enzyme, inhibitor, substrate and reference compound and comparing total ion currents (TICs) to give a measure of inhibitor effectiveness. We will also use HPLC-MS as an analysis tool in the screening of compounds cleaved from single beads. This will allow, in conjunction with automated sampling, large 'split and mix' single bead based libraries to be rapidly screened and fully analysed. The focus of these libraries will be factor viia and the caspases.
Summary
unavailable
Committee
Closed Committee - Biomolecular Sciences (BMS)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Joint Equipment Initiative 1999 (JE4) [1999]
Funding Scheme
X – not Funded via a specific Funding Scheme
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