Award details

(A) Metabolite profiling by automated HPLC-MS

Reference	JE412655
Principal Investigator / Supervisor	Professor Robert Edwards
Co-Investigators / Co-Supervisors	Professor Benjamin Davis, Professor John Gatehouse, Professor Colin Jahoda, Professor Keith Lindsey, Professor David O'Hagan, Professor Antony Slabas
Institution	Durham University
Department	Biological and Biomedical Sciences
Funding type	Research
Value (£)	60,000
Status	Completed
Type	Research Grant
Start date	30/06/2000
End date	30/12/2000
Duration	6 months

Abstract

Recent advances in instrumentation and data-management have lead to high performance liquid chromatography coupled to mass spectrometry (HPLC-MS) being the analytical method of choice when identifying ill-characterised metabolites in complex biological extracts. Coupled to this is a requirement for high throughput when screening large numbers of samples, and it is now practical to automate HPLC-MS for large scale metabolite profiling studies. We have identified a critical need for automated HPLC-MS to further progress key research programmes within Biological Sciences, as well as interdisciplinary projects being carried out by the Departments of Biological Sciences and Chemistry in: 1). exploiting phytochemical diversity in chemical synthesis; 2). defining novel biotransformations in plants and evolving novel enzyme activities; 3). defining metabolic communication between pathways in transgenic plants; 4). defining metabolic events during animal and plant development; 5). identifying novel pathways of foreign compound metabolism in plants; 6). understanding biochemical responses of plants to biotic and abiotic stress; 7). metabolic profiling of transgenic plants for pleiotropic effects of toxicological concern; and 8). defining metabolic events in plant-insect interactions.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Equipment Initiative 1999 (JE4) [1999]
Funding Scheme	X – not Funded via a specific Funding Scheme

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