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(A) Proteomic analysis of gene expression in bacteria yeasts and higher organisms

Reference	JE412607
Principal Investigator / Supervisor	Professor Ian Booth
Co-Investigators / Co-Supervisors	Dr Phillip Cash, Professor John Fothergill, Professor Neil Andrew Robert Gow
Institution	University of Aberdeen
Department	School of Medical Sciences
Funding type	Research
Value (£)	107,512
Status	Completed
Type	Research Grant
Start date	16/02/2000
End date	16/08/2000
Duration	6 months

Abstract

Proteomics offers the most rapid method for analysis of global gene expression in cells coupled with the ability to detect post-translational protein modifications that are intrinsic to many regulatory mechanisms. Proteomics is currently the only approach for the identification of post-translational modifications at a global level. Recent developments in both 2D gel technology and MALDI-TOF mass spectrometry have radically improved the power of proteomic analysis so that it is now an essential adjunct to the analysis of gene expression utilising the genome information available from a number of sequencing projects. In Aberdeen we have developed a proteomics facility that is being used by groups studying bacterial gene expression, yeast cell biology and molecular pathogenicity and biomedical groups investigating clinical conditions, e.g. diabetes, the regulation of food intake, colon cancer and bone metabolism. Keratin contamination is one of the factors that limits the sensitivity of identifying proteins by MALDI-TOF MS since it increases the background noise against which the signal is measured. Keratin contamination can be reduced very substantially by robotic technologies that allow minimal handling as well as providing a clean environment. In addition the robots will increase the rate of sample throughput in the facility, since excision of spots from the gel, digestion and clean-up are time consuming steps.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Equipment Initiative 1999 (JE4) [1999]
Funding Scheme	X – not Funded via a specific Funding Scheme

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