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(A) Regulation of gibberellin biosynthesis in plants

Reference	JE412604
Principal Investigator / Supervisor	Dr Andrew Phillips
Co-Investigators / Co-Supervisors	Professor Peter Hedden
Institution	Rothamsted Research
Department	Sustainable Soils and Grassland Systems
Funding type	Research
Value (£)	22,652
Status	Completed
Type	Research Grant
Start date	02/04/2000
End date	02/10/2000
Duration	6 months

Abstract

Gibberellins are endogenous growth regulators that are involved in all aspects of plant development. The GA-biosynthetic pathway is regulated by a range of endogenous and environmental factors including light, temperature and feedback/feedforward by bioactive GAs. We propose to use the promoter from a feedback-regulated GA-biosynthetic gene coupled to the firefly luciferase reporter gene to study the response of this promoter to different chemical and environmental stimuli, in real time. We also propose to identify components of the regulatory mechanisms by identifying mutants that affect expression of the reporter gene under a range of different experimental treatments. To detect the low level of light emitted by luciferase we require a highly-sensitive, liquid nitrogen- cooled CCD camera and image analysis software that will allow us to quantify changes in luciferase activity and to screen large numbers of mutant seedlings to identify individuals with altered responses.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Equipment Initiative 1999 (JE4) [1999]
Funding Scheme	X – not Funded via a specific Funding Scheme

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