Award details

(A) Purification of genetically labelled cells

Reference	JE412587
Principal Investigator / Supervisor	Professor Austin Smith
Co-Investigators / Co-Supervisors	Professor Clare Blackburn, Professor Alexander Medvinsky
Institution	University of Edinburgh
Department	Inst of Stem Cell Research
Funding type	Research
Value (£)	123,900
Status	Completed
Type	Research Grant
Start date	03/05/2000
End date	03/11/2000
Duration	6 months

Abstract

This proposal is to combine immunological and transgenic techniques for identifying specific cells of interest with the capability provided by flow cytometry for purification of labelled sub-populations of cells. This powerful combination of immunological, genetic and physical methodologies will be applied in particular to the cellular characterisation of mammalian stem and precursor cells. By enabling direct experimental interrogation of selected cells, this approach provides an essential contribution towards a comprehensive description of the ontogeny, properties and developmental potential of precursor cells. The propagation and manipulation of purified precursor cells in vitro can ultimately be expected to provide the foundations of systems for cell and gene therapy. In addition, the cell sorter will be available to cell biologists in the nearby Institute of Cell and Molecular Biology, and to groups at the Roslin Institute, fostering increased collaboration in the areas of transgenesis, cell differentiation and nuclear reprogramming.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Joint Equipment Initiative 1999 (JE4) [1999]
Funding Scheme	X – not Funded via a specific Funding Scheme

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